γ-Aminobutyric acid (GABA) is an amino acid found in unpolished rice, chocolate, tea, and other foods. It is an important inhibitory neurotransmitter. However, the influence of GABA on object recognition and working memory is still unknown. In this study, the effects of GABA on novel object recognition (NOR) memory and working memory were examined. The proper retention interval and delay time were also investigated for the NOR test and T-maze test, respectively. Male 3-wk-old Wistar rats were allowed free access to food and water containing 0.5% GABA or 1% GABA for a month. After that, the rats performed the NOR test at a 48 h retention interval and T-maze test at a 900 s delay time to estimate the effects of GABA on learning behavior. The results showed that the object information in the NOR test was stored as long-term memory and the recognition index (RI) was significantly increased after GABA administration. The accuracy rate also significantly increased after GABA administration. These indicate that GABA may be involved in long-term object recognition memory and working memory.