Barley stripe mosaic virus: structure and relationship to the tobamoviruses

Amy Kendall; Dewight Williams; Wen Bian; Phoebe L Stewart; Gerald Stubbs

doi:10.1016/j.virol.2013.05.010

Barley stripe mosaic virus: structure and relationship to the tobamoviruses

Virology. 2013 Sep 1;443(2):265-70. doi: 10.1016/j.virol.2013.05.010. Epub 2013 May 29.

Authors

Amy Kendall¹, Dewight Williams, Wen Bian, Phoebe L Stewart, Gerald Stubbs

Affiliation

¹ Department of Biological Sciences and Center for Structural Biology, Vanderbilt University, Nashville, TN 37235, USA.

PMID: 23725818
DOI: 10.1016/j.virol.2013.05.010

Abstract

Barley stripe mosaic virus (BSMV) is the type member of the genus Hordeivirus, rigid, rod-shaped viruses in the family Virgaviridae. We have used fiber diffraction and cryo-electron microscopy to determine the helical symmetry of BSMV to be 23.2 subunits per turn of the viral helix, and to obtain a low-resolution model of the virus by helical reconstruction methods. Features in the model support a structural relationship between the coat proteins of the hordeiviruses and the tobamoviruses.

Keywords: Barley stripe mosaic virus; Cryo-electron microscopy; Fiber diffraction; Helical reconstruction; Helical symmetry; Hordeivirus; Tobacco mosaic virus; Tobamovirus; Viral evolution.

Publication types

Research Support, N.I.H., Extramural
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

Capsid Proteins / chemistry
Capsid Proteins / genetics
Cryoelectron Microscopy
Evolution, Molecular*
Hordeum / virology*
Nicotiana / virology
RNA Viruses* / chemistry
RNA Viruses* / genetics
RNA Viruses* / ultrastructure
Tobacco Mosaic Virus* / chemistry
Tobacco Mosaic Virus* / genetics
Tobacco Mosaic Virus* / ultrastructure
X-Ray Diffraction

Substances

Capsid Proteins