The aim of this study was twofold: (a) to compare the maximal attained speed (MAS) from the 20-m shuttle (MST) and 20-m square-shuttle (SST) tests and (b) to crossvalidate 2 equations for predicting maximal oxygen consumption (VO2max) that were previously developed from MST and SST in a group of female collegiate soccer players. Thirty-nine subjects (age: 20.1 ± 1.5 years) participated in the study. A maximal graded exercise treadmill test was used to measure VO2max. In addition, VO2max was predicted from the MAS obtained during MST ((pred)VO2maxMST) and SST ((pred)VO2maxSST) using previously developed equations. Measured VO2max for the group was 44.2 ± 3.3 ml·kg(-1)·min(-1). The MAS was 12.5 ± 0.6 km·h(-1) for MST and 13.3 ± 0.8 km·h(-1) for SST (p < 0.05). The prediction methods yielded a (pred)VO2maxMST of 49.6 ± 3.9 ml·kg(-1)·min(-1) and predVO2maxSST of 41.8 ± 3.1 ml·kg(-1)·min(-1), which were significantly different from measured VO2max (p < 0.05). The validity statistics revealed the following constant error (CE), correlation coefficient (r), standard error of estimate (SEE), and total error (TE) for (pred)VO2maxMST and (pred)VO2maxSST: CE = 5.35 ± 3.83, r = 0.45 (p < 0.05), SEE = 2.97 ml·kg(-1)·min(-1), TE = 6.39 ml·kg(-1)·min(-1); and CE = -2.43 ± 2.49, r = 0.69 (p < 0.05), SEE = 2.39 ml·kg(-1)·min(-1), TE = 3.43 ml·kg(-1)·min(-1), respectively. Residual plots indicated no proportional bias for either prediction model. The results of this study suggest that female collegiate soccer players had a higher MAS from SST compared with that from MST. In addition, SST appeared to be a more accurate predictor of VO2max than MST in the group of athletes.