Purpose: To explore the feasibility of dual release baicalin and rhBMP-2 system for the treatment of periodontal diseases in minipigs.
Methods: Four miniature swines were selected to establish the model of periodontitis and randomly divided them into four groups: Dual release group, hydrogel with baicalin-GMS and rhBMP-2; Single Huang group, hydrogel with baicalin-GMS; BMP group, hydrogel with rhBMP-2; Negative control group, blank hydrogel. All parameters including clinical indicators of periodontitis, the level of IL-1 and TNF-α in the gingival crevicular fluid (GCF) were measured before and 6 weeks after modeling, and 4, 8 weeks after treatment. The data was analyzed using SPSS13.0 software package. The periodontitis model animals were sacrificed at the end of 8 weeks after treatment. The histological changes of periodontal tissues were observed under microscope with HE staining.
Results: (1)In all groups, there were significant differences of clinical indicators and levels of IL-1 and TNF-α in the GCF between periodontitis was established and 4, 8 weeks after treatment (P<0.05). Dual release group was significantly superior than that of other three groups (P<0.05). Moreover, all the parameters improved continuously. X-ray showed that bone mineral density and height in the remaining three groups increased compared with the control group, with highest in dual release group. (2)The results of histological observation showed that the inflammation reaction of periodontal tissues in negative control group was more serious than that in other groups 8 weeks after treatment. There was no obvious repair reaction in the control group, but different amount of new bone was seen in other three groups. Among them, dual release group had most obvious repair reaction.
Conclusions: The dual-slow-release chitosan thermosensitive hydrogel system is employable to be used effectively in the regeneration of periodontal defects, which laid a foundation for further clinical use.