A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis

Toshiro Kibe; Takao Fuchigami; Michiko Kishida; Mikio Iijima; Kiyohide Ishihata; Hiroshi Hijioka; Akihiko Miyawaki; Ichiro Semba; Norifumi Nakamura; Tohru Kiyono; Shosei Kishida

doi:10.1016/j.oooo.2013.03.005

A novel ameloblastoma cell line (AM-3) secretes MMP-9 in response to Wnt-3a and induces osteoclastogenesis

Oral Surg Oral Med Oral Pathol Oral Radiol. 2013 Jun;115(6):780-8. doi: 10.1016/j.oooo.2013.03.005.

Authors

Toshiro Kibe¹, Takao Fuchigami, Michiko Kishida, Mikio Iijima, Kiyohide Ishihata, Hiroshi Hijioka, Akihiko Miyawaki, Ichiro Semba, Norifumi Nakamura, Tohru Kiyono, Shosei Kishida

Affiliation

¹ Department of Biochemistry and Genetics, Kagoshima University Graduate School of Medical and Dental Sciences, Japan.

PMID: 23706918
DOI: 10.1016/j.oooo.2013.03.005

Abstract

Objective: Ameloblastoma has a high risk of bone invasion and local recurrence. However, the mechanisms of bone invasion in ameloblastoma remain unclear. In this study, we established an experimental model for matrix metalloproteinase (MMP) induction and osteoclastogenesis using ameloblastoma-derived cells.

Study design: We established an ameloblastoma-derived cell line without viral genes and analyzed the expression of all Wnt and Frizzled members and MMPs by real-time reverse transcription-polymerase chain reaction, and analyzed the activity of MMP-2 and MMP-9 by the in-gel-gelatinase assay.

Results: AM-3, newly established ameloblastoma-derived cells retained the morphology of primary-cultured ameloblastoma cells. AM-3 cells overexpressed the messenger RNA of Wnt-5a, Frizzled-2, MMP-2, and MMP-9 and showed the potential of osteoclastogenesis. In addition, Wnt-3a-treatment induced expression and activation of MMP-9 in AM-3 cells.

Conclusions: Our study suggests that AM-3 cells retained the characteristics of ameloblastoma, without acquiring typical features of cancer cells. Furthermore, Wnt signaling induced MMP-9 in ameloblastoma cells.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Ameloblastoma / metabolism*
Cell Line, Tumor / metabolism
Epithelial-Mesenchymal Transition / genetics
Epithelial-Mesenchymal Transition / physiology*
Frizzled Receptors / genetics
Frizzled Receptors / metabolism*
Gene Expression
Humans
Jaw Neoplasms / genetics
Jaw Neoplasms / metabolism*
Matrix Metalloproteinase 9 / metabolism*
Osteoclasts / metabolism*
RNA, Messenger / metabolism
Reverse Transcriptase Polymerase Chain Reaction
Wnt Signaling Pathway / physiology*

Substances

Frizzled Receptors
RNA, Messenger
Matrix Metalloproteinase 9