Objective: To establish the fingerprint of Polygonum multiflorum from Guizhou and provide a standard for its quality control.
Methods: HPLC analysis was performed on Agillent ZABAX-C18 (4.6 mm x 250 mm, 5 microm), gradient eluted composed of acetonitrile-0.4% water solution of phosphoric acid. Column temperature was set at 25 degrees C and the flow rate was 1 mL/min. The detection wavelength was 280 nm and the analysis time was 60 min.
Results: 9 common peaks were identified. The RSD of the relative retention time and the relative peak area were less than 3% in analyzing its precision, stability and repeatability of the common peaks, and the similarity of the 16 batches of sample was more than 0.9.
Conclusion: The method is simple and reliable, and it can provide a standard and guidance for quality control of Polygonum multiflorum.