Abstract
To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Azo Compounds / chemistry
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Azo Compounds / metabolism*
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Base Sequence
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Conserved Sequence / genetics
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Fluorescence
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Genes, Viral / genetics*
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Genome, Viral / genetics
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Immobilized Proteins / metabolism
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Orthomyxoviridae / genetics*
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Peptide Nucleic Acids / chemistry
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Peptide Nucleic Acids / metabolism*
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Protein Binding
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RNA, Viral / genetics
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Viral Nonstructural Proteins / genetics*
Substances
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Azo Compounds
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Immobilized Proteins
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NS protein, influenza virus
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Peptide Nucleic Acids
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RNA, Viral
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Viral Nonstructural Proteins