Sequence-specific and visual identification of the influenza virus NS gene by azobenzene-tethered bis-peptide nucleic acid

PLoS One. 2013 May 21;8(5):e64017. doi: 10.1371/journal.pone.0064017. Print 2013.

Abstract

To rapidly and specifically identify highly virulent influenza virus strains, we prepared an azobenzene-tethered hairpin-type peptide nucleic acid, bisPNA-AZO, which has a complementary sequence against a highly conserved genomic RNA sequence within the ribonucleoprotein complex of the 2009 pandemic influenza A virus, H1N1 subtype. bisPNA-AZO recognizes the conserved virus genome sequence in a sequence-specific manner. Immobilization of bisPNA-AZO on a plate allowed capture of the target virus gene and the generation of a visual colour signal.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Azo Compounds / chemistry
  • Azo Compounds / metabolism*
  • Base Sequence
  • Conserved Sequence / genetics
  • Fluorescence
  • Genes, Viral / genetics*
  • Genome, Viral / genetics
  • Immobilized Proteins / metabolism
  • Orthomyxoviridae / genetics*
  • Peptide Nucleic Acids / chemistry
  • Peptide Nucleic Acids / metabolism*
  • Protein Binding
  • RNA, Viral / genetics
  • Viral Nonstructural Proteins / genetics*

Substances

  • Azo Compounds
  • Immobilized Proteins
  • NS protein, influenza virus
  • Peptide Nucleic Acids
  • RNA, Viral
  • Viral Nonstructural Proteins