Efficacy of polymer coating of probiotic beads suspended in pressurized and pasteurized longan juices on the exposure to simulated gastrointestinal environment

Int J Food Sci Nutr. 2013 Nov;64(7):862-9. doi: 10.3109/09637486.2013.799124. Epub 2013 May 24.

Abstract

Alginate-coated Lactobacillus acidophilus LA5 or Lactobacillus casei 01 was recoated with either 0.1-0.5% (w/v) alginate or 0.05-0.15% (w/v) poly-L-lysine (PLL) plus 0.2% (w/v) alginate or 5-15% (w/v) gelatin, after which they were determined for survivability in gastric or bile longan juices. The morphology of encapsulated probiotic cells illustrated that recoated beads with 0.5% alginate showed a more compact surface and a greater protective effect than other recoating materials. The recoated beads with 0.5% alginate and 0.05-0.15% PLL plus 0.2% alginate of both strains showed the highest viability in gastric longan juice. In bile longan juice, only 0.5% alginate showed the best protection for both recoated beads. When considering the storage stability, encapsulated L. acidophilus LA5 exhibited a higher viable count than those of the free cells, whereas L. casei 01 showed equivalent viability of both free and double-coated cells. Based on the impact of pressurization or pasteurization, both processed juices gave rise to equivalent survivability of the probiotic cells during storage.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alginates
  • Bile*
  • Capsules*
  • Gastric Juice*
  • Gastrointestinal Tract*
  • Lacticaseibacillus casei
  • Lactobacillus acidophilus
  • Lysine
  • Microbial Viability*
  • Pasteurization
  • Polylysine / analogs & derivatives
  • Polymers*
  • Pressure
  • Probiotics / administration & dosage*
  • Surface Properties

Substances

  • Alginates
  • Capsules
  • Polymers
  • alginate-polylysine-alginate
  • Polylysine
  • Lysine