Effect of olive leaf (Olea europea L.) extracts on protein and lipid oxidation in cooked pork meat patties enriched with n-3 fatty acids

J Sci Food Agric. 2014 Jan 30;94(2):227-34. doi: 10.1002/jsfa.6236. Epub 2013 Jun 24.

Abstract

Background: The effect of olive leaf extracts on lipid and protein oxidation of cooked pork patties refrigerated stored for 9 days was evaluated. Patties were prepared from longissimus dorsi muscle of pigs, and dietary supplemented with linseed oil.

Results: Results showed that dietary linseed oil modified the fatty acid composition of pork patties by increasing (P ≤ 0.05) n-3 (α-linolenic acid) and decreasing (P ≤ 0.05) n-6 (linoleic acid) fatty acids. Olive leaf extracts at supplementation levels of 200 and, especially, of 300 mg gallic acid equivalents kg⁻¹ meat, delayed lipid oxidation by reducing (P ≤ 0.05) both primary (conjugated dienes and hydroperoxides) and secondary (malondialdehyde) oxidation products. They also inhibited protein oxidation in a concentration-dependent manner by reducing (P ≤ 0.05) protein carbonyls and increasing (P ≤ 0.05) protein sulfhydryls. In addition, they improved sensory attributes of the n-3 enriched patties.

Conclusion: Results suggested that olive leaf extracts might be useful to the meat industry as an efficient alternative to synthetic antioxidants.

Keywords: lipid oxidation; n-3 pork patties; olive leaf extracts; protein oxidation; α-tocopherol.

MeSH terms

  • Animals
  • Antioxidants
  • Cooking
  • Diet
  • Dietary Fats / metabolism
  • Dietary Fats / pharmacology
  • Dietary Proteins / metabolism*
  • Dietary Supplements
  • Fatty Acids, Omega-3 / metabolism*
  • Fatty Acids, Omega-3 / pharmacology
  • Fatty Acids, Omega-6 / metabolism
  • Flax / chemistry
  • Food, Fortified
  • Linseed Oil / metabolism
  • Linseed Oil / pharmacology*
  • Lipid Peroxidation*
  • Meat / analysis*
  • Muscle, Skeletal
  • Olea*
  • Plant Extracts
  • Plant Leaves
  • Protein Carbonylation*
  • Swine

Substances

  • Antioxidants
  • Dietary Fats
  • Dietary Proteins
  • Fatty Acids, Omega-3
  • Fatty Acids, Omega-6
  • Plant Extracts
  • Linseed Oil