Morphology of two marine euplotids (Ciliophora: Euplotida), Aspidisca fusca and A. hexeris, with notes on their small subunit rRNA gene sequences

Abstract

We investigate the living morphology, infraciliature, and small subunit rRNA gene sequences of two poorly known marine euplotids, Aspidisca fuscaKahl, 1928 and A. hexerisQuennerstedt, 1869, isolated from intertidal sewage outfall, northern China and sandy sediments, southern China, respectively. Improved diagnoses and morphometric data are provided for both species. Aspidisca fusca is characterized by having a peristomial spur, seven frontoventral cirri in a "polystyla-arrangement", and two oval macronuclear nodules. Aspidisca hexeris can be recognized by having an elliptical body shape, a peristomial spur, seven membranelles in the anterior portion of adoral zone and 11-13 in the posterior part, as well as seven frontoventral cirri arranged in two oblique rows. In addition, the presence and degree of projections along the left margin of A. hexeris are highly variable among populations. Phylogenetic analyses based on small subunit rRNA gene sequence data support the validity of both A. hexeris and A. fusca as distinct species, and indicate that A. fusca is most closely related to A. aculeata and A. steini, whereas A. hexeris shows a close relationship with A. magna.

Keywords: Benthos; China; Ciliate; Euplotia; Phylogeny; Taxonomy.