The porcine brucellosis--evidence of the role of Yersinia enterocolitica O:9 in occurrence of false positive serological reactions

Pol J Vet Sci. 2013;16(1):129-30. doi: 10.2478/pjvs-2013-0019.

Abstract

Forty four pigs with typical characteristics for false positive serolpgical reactions (FPSR) were examined for the presence of Yersinia enterocolitica O:9. The positive reactions were observed in rose bengal test (RBT, N = 23 sera), serum agglutination test (SAT, N = 16), complement fixation test (CFT, N = 9), indirect ELISA (i-ELISA, N= 11) in first, and in RBT (N = 14), SAT (N = 8), CFT (N = 7) and i-ELISA (N = 18) in second examination, respectively. In bacteriological examination Y enterocolitica was confirmed in 12 cases. Six of these isolates were identified with PCR as Y enterocolitica 0:9.

MeSH terms

  • Animals
  • Brucellosis / diagnosis
  • Brucellosis / veterinary*
  • False Positive Reactions
  • Female
  • RNA, Bacterial / genetics
  • RNA, Ribosomal, 16S / genetics
  • Serologic Tests / veterinary*
  • Swine
  • Swine Diseases / diagnosis*
  • Swine Diseases / microbiology
  • Yersinia Infections / diagnosis
  • Yersinia Infections / genetics
  • Yersinia Infections / microbiology
  • Yersinia Infections / veterinary*
  • Yersinia enterocolitica*

Substances

  • RNA, Bacterial
  • RNA, Ribosomal, 16S