Single-molecule microscopy of the green fluorescent protein using simultaneous two-color excitation

G Jung; J Wiehler; B Steipe; C Bräuchle; A Zumbusch

doi:10.1002/1439-7641(20010618)2:6<392::AID-CPHC392>3.0.CO;2-7

Single-molecule microscopy of the green fluorescent protein using simultaneous two-color excitation

Chemphyschem. 2001 Jun 18;2(6):392-6. doi: 10.1002/1439-7641(20010618)2:6<392::AID-CPHC392>3.0.CO;2-7.

Authors

G Jung¹, J Wiehler, B Steipe, C Bräuchle, A Zumbusch

Affiliation

¹ Department Chemie, Lehrstuhl Physikalische Chemie I, Ludwig-Maximilians Universität München, München, Germany.

PMID: 23686962
DOI: 10.1002/1439-7641(20010618)2:6<392::AID-CPHC392>3.0.CO;2-7

Abstract

In vivo microscopy of the Green Fluorescent Protein (GFP), the most important label in cell biology, with single-molecule sensitivity is hampered by an insufficient signal-to-noise ratio. A significant improvement is obtained with a novel two-color excitation technique. The picture clearly shows the increased brightness of GFP in in vitro single-molecule assays and in live-cell microscopy under two-color illumination (upper cell) as compared to normal illumination (lower cell).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Color*
Green Fluorescent Proteins / chemistry*
Spectrometry, Fluorescence
Spectrophotometry, Ultraviolet

Substances

Green Fluorescent Proteins