Abstract
Two new β-carboline alkaloids, variabines A (1) and B (2), were isolated from the Indonesian marine sponge Luffariella variabilis. Their structures were elucidated from spectral data, and 1 was found to be a sulfonated derivative of 2. Although numerous β-carboline alkaloids have been isolated from natural sources to date, 1 is the first β-carboline derivative containing a sulfate group. Compound 2 inhibited chymotrypsin-like activity of the proteasome and Ubc13 (E2)-Uev1A interaction with IC50 values of 4 and 5 μg/mL, respectively, whereas 1 had little effect on the activity or interaction.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaloids / isolation & purification*
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Alkaloids / pharmacology
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Animals
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Carbolines / isolation & purification*
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Carbolines / pharmacology
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Humans
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Liver / drug effects
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Liver / enzymology
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Molecular Structure
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Porifera / chemistry*
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Proteasome Inhibitors / isolation & purification
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Proteasome Inhibitors / pharmacology*
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Rats
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Transcription Factors / antagonists & inhibitors
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Transcription Factors / metabolism
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Ubiquitin-Conjugating Enzymes / antagonists & inhibitors
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Ubiquitin-Conjugating Enzymes / metabolism
Substances
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Alkaloids
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Carbolines
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Proteasome Inhibitors
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Transcription Factors
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variabine A
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variabine B
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UBE2N protein, human
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UBE2V1 protein, human
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Ubiquitin-Conjugating Enzymes