Abstract
Ginsenoside Rb2 was transformed by recombinant glycosidase (Bgp2) into ginsenosides Rd and 20(S)-Rg3. The bgp2 gene consists of 2,430 bp that encode 809 amino acids, and this gene has homology to the glycosyl hydrolase family 2 protein domain. SDS-PAGE was used to determine that the molecular mass of purified Bgp2 was 87 kDa. Using 0.1 mg ml(-1) of enzyme in 20 mM sodium phosphate buffer at 40 °C and pH 7.0, 1.0 mg ml(-1) ginsenoside Rb2 was transformed into 0.47 mg ml(-1) ginsenoside 20(S)-Rg3 within 120 min, with a corresponding molar conversion yield of 65 %. Bgp2 hydrolyzed the ginsenoside Rb2 along the following pathway: Rb2 → Rd → 20(S)-Rg3. This is the first report of the biotransformation of ginsenoside Rb2 to ginsenoside 20(S)-Rg3 using the recombinant glycosidase.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Actinomycetales / enzymology*
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Actinomycetales / genetics
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Bacterial Proteins / genetics
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Bacterial Proteins / isolation & purification*
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Bacterial Proteins / metabolism
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Biotransformation
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Chromatography, Thin Layer
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Cloning, Molecular
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Ginsenosides / biosynthesis
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Ginsenosides / metabolism*
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Glycoside Hydrolases / genetics
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Glycoside Hydrolases / isolation & purification*
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Glycoside Hydrolases / metabolism
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Hydrogen-Ion Concentration
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Hydrolysis
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Mass Spectrometry
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Molecular Structure
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Recombinant Fusion Proteins / isolation & purification
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Recombinant Fusion Proteins / metabolism
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Substrate Specificity
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Temperature
Substances
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Bacterial Proteins
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Ginsenosides
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Recombinant Fusion Proteins
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ginsenoside Rb2
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ginsenoside Rg3
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Glycoside Hydrolases
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ginsenoside Rd