Rapid detection of human cytomegalovirus UL97 and UL54 mutations for antiviral resistance in clinical specimens

Microbiol Immunol. 2013 May;57(5):396-9. doi: 10.1111/1348-0421.12043.

Abstract

Drug-resistant cytomegalovirus appears during prolonged anti-cytomegalovirus therapy. Assays for human cytomegalovirus viral protein kinase (UL97) and viral DNA polymerase (UL54) gene mutations conferring drug resistance have been used rather than susceptibility assays to assess clinical specimens. In this study a sensitive system for genotype assay of UL97 and UL54 in clinical specimens with as few as six copies/µg of DNA was developed.

Publication types

  • Evaluation Study

MeSH terms

  • Adult
  • Antiviral Agents / pharmacology*
  • Child
  • Cytomegalovirus / drug effects*
  • Cytomegalovirus / genetics
  • Cytomegalovirus / isolation & purification
  • Cytomegalovirus Infections / virology
  • DNA-Directed DNA Polymerase / genetics*
  • Drug Resistance, Viral*
  • Humans
  • Microbial Sensitivity Tests / methods
  • Middle Aged
  • Molecular Diagnostic Techniques / methods*
  • Mutation, Missense*
  • Phosphotransferases (Alcohol Group Acceptor) / genetics*
  • Sensitivity and Specificity
  • Viral Proteins / genetics*

Substances

  • Antiviral Agents
  • UL54 protein, Human herpesvirus 5
  • Viral Proteins
  • Phosphotransferases (Alcohol Group Acceptor)
  • ganciclovir kinase
  • DNA-Directed DNA Polymerase