Abstract
Drug-resistant cytomegalovirus appears during prolonged anti-cytomegalovirus therapy. Assays for human cytomegalovirus viral protein kinase (UL97) and viral DNA polymerase (UL54) gene mutations conferring drug resistance have been used rather than susceptibility assays to assess clinical specimens. In this study a sensitive system for genotype assay of UL97 and UL54 in clinical specimens with as few as six copies/µg of DNA was developed.
© 2013 The Societies and Wiley Publishing Asia Pty Ltd.
MeSH terms
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Adult
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Antiviral Agents / pharmacology*
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Child
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Cytomegalovirus / drug effects*
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Cytomegalovirus / genetics
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Cytomegalovirus / isolation & purification
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Cytomegalovirus Infections / virology
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DNA-Directed DNA Polymerase / genetics*
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Drug Resistance, Viral*
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Humans
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Microbial Sensitivity Tests / methods
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Middle Aged
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Molecular Diagnostic Techniques / methods*
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Mutation, Missense*
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Phosphotransferases (Alcohol Group Acceptor) / genetics*
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Sensitivity and Specificity
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Viral Proteins / genetics*
Substances
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Antiviral Agents
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UL54 protein, Human herpesvirus 5
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Viral Proteins
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Phosphotransferases (Alcohol Group Acceptor)
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ganciclovir kinase
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DNA-Directed DNA Polymerase