Expression and immunological characterization of cardamom mosaic virus coat protein displaying HIV gp41 epitopes

Microbiol Immunol. 2013 May;57(5):374-85. doi: 10.1111/1348-0421.12045.

Abstract

The coat protein of cardamom mosaic virus (CdMV), a member of the genus Macluravirus, assembles into virus-like particles when expressed in an Escherichia coli expression system. The N and C-termini of the coat protein were engineered with the Kennedy peptide and the 2F5 and 4E10 epitopes of gp41 of HIV. The chimeric proteins reacted with sera from HIV positive persons and also stimulated secretion of cytokines by peripheral blood mononuclear cells from these persons. Thus, a system based on the coat protein of CdMV can be used to display HIV-1 antigens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Capsid Proteins / genetics
  • Capsid Proteins / immunology*
  • Cytokines / metabolism*
  • Drug Carriers*
  • Epitopes / genetics
  • Epitopes / immunology*
  • HIV Antibodies / blood*
  • HIV Envelope Protein gp41 / genetics
  • HIV Envelope Protein gp41 / immunology*
  • Leukocytes, Mononuclear / immunology*
  • Potyviridae / genetics
  • Recombinant Fusion Proteins / genetics
  • Recombinant Fusion Proteins / immunology

Substances

  • Capsid Proteins
  • Cytokines
  • Drug Carriers
  • Epitopes
  • HIV Antibodies
  • HIV Envelope Protein gp41
  • Recombinant Fusion Proteins
  • gp41 protein, Human immunodeficiency virus 1