Abstract
Essential oil from Cymbopogon nardus was evaluated for activity against Trypanosoma brucei brucei BS221 (IC50 = 0.31 ± 0.03 μg/mL) and cytotoxic effect on normal kidney (Vero) cells (IC50 = >100 μg/mL). The crude essential oil was subjected to various chromatography techniques afforded active sub fractions with antitrypanosomal activity; F4 (IC50 = 0.61 ± 0.06 μg/mL), F6 (IC50= 0.73 ± 0.33 μg/mL), F7 (IC50 = 1.15 ± 0 μg/mL) and F8 (IC50 = 1.11 ± 0.01 μg/mL). These active fractions did not exhibit any toxic effects against Vero cell lines and the chemical profiles investigation indicated presence of α-and γ-eudesmol, elemol, α-cadinol and eugenol by GC/MS analysis.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antiprotozoal Agents / chemistry
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Antiprotozoal Agents / isolation & purification
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Antiprotozoal Agents / pharmacology*
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Antiprotozoal Agents / toxicity
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Cell Survival / drug effects
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Chlorocebus aethiops
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Cymbopogon / chemistry*
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Epithelial Cells / drug effects
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Epithelial Cells / physiology
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Gas Chromatography-Mass Spectrometry
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Inhibitory Concentration 50
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Oils, Volatile / chemistry
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Oils, Volatile / isolation & purification
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Oils, Volatile / pharmacology*
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Oils, Volatile / toxicity
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Parasitic Sensitivity Tests
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Plant Extracts / chemistry
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Plant Extracts / isolation & purification
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Plant Extracts / pharmacology*
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Plant Extracts / toxicity
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Trypanosoma brucei brucei / drug effects*
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Vero Cells
Substances
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Antiprotozoal Agents
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Oils, Volatile
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Plant Extracts