In the present work we develop a novel approach for quantification of the energetics of C60 fullerene aggregation in aqueous media in terms of equilibrium aggregation constant KF. In particular, it is shown that the experimental determination of the magnitude of KF is possible only within the framework of the 'up-scaled aggregation model', considering the C60 fullerene water solution as a solution of fullerene clusters. Using dynamic light scattering (DLS) data we report the value, K(F) = 56,000 M(-1), which is in good agreement with existing theoretical estimates and the results of energetic analyses. It is suggested that the proposed 'up-scaled model' may be used in any instances of non-specific aggregation resulting in formation of large spherical particles. The measurement of the translational diffusion coefficient and the dimensions of the light scattering particles using a DLS approach with respect to C60 fullerene aggregates is found to contain significant systematic errors originating from the interaction effect that is well-known for micellar solutions. As a result, corrections to the equations associated with DLS data are proposed.