Abstract
Macrolides are an important tool in the treatment of Mycobacterium avium complex infections. Here, we evaluate the use of 23S rRNA gene sequencing for the rapid detection of macrolide resistance. Routine sequencing of the 23S rRNA gene is highly specific for macrolide resistance but lacks in sensitivity.
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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DNA, Bacterial / chemistry
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DNA, Bacterial / genetics
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DNA, Ribosomal / chemistry
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DNA, Ribosomal / genetics
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Drug Resistance, Bacterial*
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Humans
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Macrolides / pharmacology*
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Microbial Sensitivity Tests / methods
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Molecular Diagnostic Techniques / methods*
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Mycobacterium avium Complex / drug effects*
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Mycobacterium avium Complex / genetics
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RNA, Ribosomal, 23S / genetics
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Sensitivity and Specificity
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Sequence Analysis, DNA / methods
Substances
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Anti-Bacterial Agents
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DNA, Bacterial
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DNA, Ribosomal
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Macrolides
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RNA, Ribosomal, 23S