Rapid molecular detection of macrolide resistance in the Mycobacterium avium complex: are we there yet?

J Clin Microbiol. 2013 Jul;51(7):2425-6. doi: 10.1128/JCM.00555-13. Epub 2013 May 8.

Abstract

Macrolides are an important tool in the treatment of Mycobacterium avium complex infections. Here, we evaluate the use of 23S rRNA gene sequencing for the rapid detection of macrolide resistance. Routine sequencing of the 23S rRNA gene is highly specific for macrolide resistance but lacks in sensitivity.

Publication types

  • Evaluation Study

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • Drug Resistance, Bacterial*
  • Humans
  • Macrolides / pharmacology*
  • Microbial Sensitivity Tests / methods
  • Molecular Diagnostic Techniques / methods*
  • Mycobacterium avium Complex / drug effects*
  • Mycobacterium avium Complex / genetics
  • RNA, Ribosomal, 23S / genetics
  • Sensitivity and Specificity
  • Sequence Analysis, DNA / methods

Substances

  • Anti-Bacterial Agents
  • DNA, Bacterial
  • DNA, Ribosomal
  • Macrolides
  • RNA, Ribosomal, 23S