Evaluation of Simplexa Flu A/B & RSV for direct detection of influenza viruses (A and B) and respiratory syncytial virus in patient clinical samples

J Clin Microbiol. 2013 Jul;51(7):2421-4. doi: 10.1128/JCM.00286-13. Epub 2013 May 8.

Abstract

We evaluated the performance of the Simplexa Flu A/B & RSV kit on 170 prospective respiratory samples using a modified protocol, supplied by the manufacturer, that eliminates the RNA extraction step. Overall, compared against our laboratory-developed assay, the assay's sensitivity, specificity, and positive and negative predictive values were 95.1%, 99.6%, 98.7%, and 98.6%, respectively.

MeSH terms

  • Humans
  • Influenza, Human / diagnosis*
  • Influenza, Human / virology
  • Molecular Diagnostic Techniques / methods*
  • Orthomyxoviridae / classification
  • Orthomyxoviridae / genetics
  • Orthomyxoviridae / isolation & purification*
  • RNA, Viral / genetics
  • RNA, Viral / isolation & purification
  • Respiratory Syncytial Virus Infections / diagnosis*
  • Respiratory Syncytial Virus Infections / virology
  • Respiratory Syncytial Virus, Human / genetics
  • Respiratory Syncytial Virus, Human / isolation & purification*
  • Retrospective Studies
  • Sensitivity and Specificity
  • Specimen Handling / methods*
  • Virology / methods*

Substances

  • RNA, Viral