Abstract
In this work, we evaluated blocking properties of various reagents used in dot immunoassay test systems on nitrocellulose membranes employing carbon-based diagnosticum to determine antibodies to tetanus anatoxin, thermostable toxin of Yersinia pseudotuberculosis, and HIV-I. The results support the usage of a nonionic detergent Tween 20 which efficiently inhibited nonspecific protein binding to the solid-phase reagent surface and did not decrease sensitivity of the analysis.
MeSH terms
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Antibodies, Viral / analysis
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Antibodies, Viral / chemistry
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Antibodies, Viral / immunology
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Artifacts
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Bacterial Toxins / analysis
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Bacterial Toxins / chemistry
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Bacterial Toxins / immunology
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HIV-1 / immunology
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Immobilized Proteins / chemistry
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Immunoblotting / methods*
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Indicators and Reagents / chemistry
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Ligands
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Protein Binding
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Yersinia pseudotuberculosis / immunology
Substances
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Antibodies, Viral
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Bacterial Toxins
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Immobilized Proteins
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Indicators and Reagents
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Ligands