Interest in berries from South America has increased due to their potential health benefits. The objective of this study was to characterize the anthocyanins and proanthocyanidins of Vaccinium floribundum and Aristotelia chilensis , total phenolics, and antioxidant capacity and to evaluate, in vitro, the ability of their phenolic extracts to reduce adipogenesis and lipid accumulation in 3T3-L1 adipocytes. The anti-inflammatory property of these extracts on RAW 264.7 macrophages was also investigated. Antioxidant capacity, measured as oxygen radical scavenging capacity and expressed as Trolox equivalents, was higher in the berries of A. chilensis. Phenolic extracts inhibited lipid accumulation by 4.0-10.8% when adipocytes were treated at maturity and by 5.9-37.9% when treated throughout differentiation. Furthermore, a proanthocyanidin-enriched fraction from V. floribundum significantly increased Pref-1 expression in preadipocytes. Phenolic extracts decreased the production of nitric oxide (3.7-25.5%) and prostaglandin E2 (9.1-89.1%) and the expression of inducible nitric oxide synthase (9.8-61.8%) and cycloxygenase-2 (16.6-62.0%) in lipopolysaccharide-stimulated RAW 264.7 macrophages. V. floribundum and A. chilensis phytochemicals limit adipogenesis and inflammatory pathways in vitro, warranting further in vivo studies.