GC models for separation optimization in pressure-tuneable tandem capillary columns operated isothermally. Part 1: Theoretical aspects

Enrico Veschetti; Ivan Notardonato; Pasquale Avino; Mario Vincenzo Russo

doi:10.1002/jssc.201300233

GC models for separation optimization in pressure-tuneable tandem capillary columns operated isothermally. Part 1: Theoretical aspects

J Sep Sci. 2013 Jul;36(14):2260-7. doi: 10.1002/jssc.201300233.

Authors

Enrico Veschetti¹, Ivan Notardonato, Pasquale Avino, Mario Vincenzo Russo

Affiliation

¹ Istituto Superiore di Sanità, Dipartimento Ambiente e Connessa Prevenzione Primaria, Rome, Italy.

PMID: 23640871
DOI: 10.1002/jssc.201300233

Abstract

GC column selectivity can be continuously adapted to suit analytical needs using a flow-tuneable tandem system. Its application for the separation of complex mixtures requires a deep understanding of the theory in this area. Although a number of researchers have developed specific models, a general and exhaustive theory is still missing. In this paper, we have made an implementation of pre-existing models on tandem-column assemblies operated isothermally. In particular, we have investigated the effect of column length and diameter, phase thickness, and oven temperature on chromatographic parameters, such as capacity factor, selectivity, and intrinsic resolution. A new approach for the correct choice of the working temperature has been proposed.

Keywords: Isothermal gas chromatography; Selectivity tuning; Tandem-column chromatography; Temperature optimization; Theory.