Molecular cloning and sequence analysis of a phenylalanine ammonia-lyase gene from dendrobium

PLoS One. 2013 Apr 30;8(4):e62352. doi: 10.1371/journal.pone.0062352. Print 2013.

Abstract

In this study, a phenylalanine ammonia-lyase (PAL) gene was cloned from Dendrobium candidum using homology cloning and RACE. The full-length sequence and catalytic active sites that appear in PAL proteins of Arabidopsis thaliana and Nicotiana tabacum are also found: PAL cDNA of D. candidum (designated Dc-PAL1, GenBank No. JQ765748) has 2,458 bps and contains a complete open reading frame (ORF) of 2,142 bps, which encodes 713 amino acid residues. The amino acid sequence of DcPAL1 has more than 80% sequence identity with the PAL genes of other plants, as indicated by multiple alignments. The dominant sites and catalytic active sites, which are similar to that showing in PAL proteins of Arabidopsis thaliana and Nicotiana tabacum, are also found in DcPAL1. Phylogenetic tree analysis revealed that DcPAL is more closely related to PALs from orchidaceae plants than to those of other plants. The differential expression patterns of PAL in protocorm-like body, leaf, stem, and root, suggest that the PAL gene performs multiple physiological functions in Dendrobium candidum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • Dendrobium / chemistry
  • Dendrobium / enzymology*
  • Dendrobium / genetics
  • Models, Molecular
  • Molecular Sequence Data
  • Phenylalanine Ammonia-Lyase / analysis
  • Phenylalanine Ammonia-Lyase / genetics*
  • Phylogeny
  • Sequence Alignment

Substances

  • Phenylalanine Ammonia-Lyase

Associated data

  • GENBANK/JQ765748

Grants and funding

The study was supported by The Key Project of Anhui Education Department (No. KJ2011A103,KJ2012A121, www.ahedu.gov.cn) and Youth Foundation of Anhui Natural Science Fund (No. 10040606Q22, www.ahkjt.gov.cn). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.