A cellular logic circuit for the detection of bacterial pore-forming toxins

Chem Commun (Camb). 2013 Jun 7;49(45):5198-200. doi: 10.1039/c3cc41932k.

Abstract

We present a cellular logic circuit for deciphering the profiles of toxin production in B. cereus, using multiple readout techniques based on the pore formation on the cell membrane. This new assay enables the simultaneous detection of seven biomarkers in pathogenic strains from various samples.

MeSH terms

  • Animals
  • Bacillus cereus / metabolism*
  • Bacterial Toxins / analysis*
  • Bacterial Toxins / metabolism
  • Bacterial Toxins / toxicity*
  • Biosensing Techniques / instrumentation*
  • Cell Membrane / microbiology*
  • Cell Survival
  • Chlorocebus aethiops
  • Equipment Design
  • Toxicity Tests / instrumentation*
  • Vero Cells

Substances

  • Bacterial Toxins