Antibiotic prophylaxis has been a matter of great interest and has been discussed by researchers and clinicians over the years. Changes and recommendations have been made in order to clarify protocol and make it safer for patients. Various organizations such as the American Heart Association, American Academy of Oral Surgeons, American Dental Association, and American Academy of Oral Medicine have played vital roles in formulating guidelines for antibiotic prophylaxis. These recommendations for prevention of infective endocarditis have been based on clinical experiences and research as well as expert opinion. This paper summarizes the most recent guidelines for general readers so that informed decisions may be made that are in the interest of patients and practitioners.