The complete mitogenome was first determined for a minnow Pseudohemiculter dispar in this study. It is 16,621 bp in length, including 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and 1 control region. Overall nucleotide base composition is 30.30% A, 27.10% C, 17.10% G, and 25.50% T, with a A+T bias. Except for the tRNA-Ser2 that lost the dihydrouridine arm and replaced with one 11-bp loop, the other 22 tRNA genes can fold into the typical cloverleaf secondary structures. The origin of light-strand replication (OL) was found between the tRNA-Asn and tRNA-Cys genes, while the termination-associated sequence (TAS) was found in the control region. Both OL and TAS can form the hairpin structure that acts as the signals participated in the mitochondrial DNA replication.