Detection of specific antibodies against tembusu virus in ducks by use of an E protein-based enzyme-linked immunosorbent assay

J Clin Microbiol. 2013 Jul;51(7):2400-2. doi: 10.1128/JCM.00361-13. Epub 2013 Apr 24.

Abstract

We developed an enzyme-linked immunosorbent assay (ELISA) using eukaryotically expressed E protein as the antigen (termed E-ELISA) to detect antibodies to tembusu virus (TMUV) in ducks. The E-ELISA did not react with antisera to other known pathogens, indicating the E protein is specific for recognizing anti-TMUV antibodies. Compared to the serum neutralization test, the specificity and sensitivity of the E-ELISA was 93.2 and 97.8%, respectively. Therefore, this E-ELISA is a sensitive and rapid method for detecting antibodies against TMUV in ducks.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Antigens, Viral*
  • Ducks
  • Enzyme-Linked Immunosorbent Assay / methods
  • Flavivirus / immunology*
  • Flavivirus Infections / immunology
  • Flavivirus Infections / veterinary*
  • Flavivirus Infections / virology
  • Sensitivity and Specificity
  • Veterinary Medicine / methods*
  • Viral Envelope Proteins*
  • Virology / methods*

Substances

  • Antibodies, Viral
  • Antigens, Viral
  • Viral Envelope Proteins
  • glycoprotein E, Flavivirus