Development of a MALDI-TOF MS strategy for the high-throughput analysis of biomarkers: on-target aptamer immobilization and laser-accelerated proteolysis

Xueyang Zhang; Shaochun Zhu; Ya Xiong; Chunhui Deng; Xiangmin Zhang

doi:10.1002/anie.201300566

Development of a MALDI-TOF MS strategy for the high-throughput analysis of biomarkers: on-target aptamer immobilization and laser-accelerated proteolysis

Angew Chem Int Ed Engl. 2013 Jun 3;52(23):6055-8. doi: 10.1002/anie.201300566. Epub 2013 Apr 22.

Authors

Xueyang Zhang¹, Shaochun Zhu, Ya Xiong, Chunhui Deng, Xiangmin Zhang

Affiliation

¹ Department of Chemistry and Institute of Biomedical Science, Fudan University, 220 Handan Road, Shanghai, 200433, China.

PMID: 23610069
DOI: 10.1002/anie.201300566

Abstract

An aptamer-based strategy was developed for the high-throughput analysis of protein biomarkers, such as lysozyme, by on-target MALDI-TOF MS. The aptamers were immobilized on the target plate through formation of covalent bonds with a stable and porous gold layer. An infrared laser was subsequently applied for fast proteolysis. High sensitivities were observed both in standard solutions and human urine.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aptamers, Peptide
Biomarkers
Gold / chemistry*
High-Throughput Screening Assays
Humans
Proteolysis
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*

Substances

Aptamers, Peptide
Biomarkers
Gold