Abstract
ICOSL, a newly identified member of the B7 superfamily, plays a major role in immune responses. In this study, a functional anti-human ICOSL monoclonal antibody (MAb) 3B3 was obtained and characterized by means of flow cytometry, Western blot, and competition assay. This MAb could specifically recognize a distinct epitope of the ICOSL molecule. As a functional antibody, MAb 3B3 could inhibit the proliferation of T lymphocytes stimulated by ICOSL-L929 transfectants. Furthermore, it could enhance IgG production of PWM-driven B cells. The results indicate that the ICOS-ICOSL signal is critically involved in specific humoral immunity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Monoclonal, Murine-Derived / pharmacology*
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B-Lymphocytes / immunology*
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B-Lymphocytes / metabolism
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Binding, Competitive
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Blotting, Western
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Cell Line, Tumor
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Cell Proliferation
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Epitope Mapping
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Humans
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Hybridomas
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Immunity, Humoral
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Immunoglobulin G / biosynthesis*
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Immunoglobulin G / immunology
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Inducible T-Cell Co-Stimulator Ligand / antagonists & inhibitors
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Inducible T-Cell Co-Stimulator Ligand / immunology*
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Inducible T-Cell Co-Stimulator Ligand / metabolism
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Mice
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Mice, Inbred BALB C
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Pokeweed Mitogens / immunology
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Pokeweed Mitogens / pharmacology
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Protein Binding
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T-Lymphocytes / drug effects
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T-Lymphocytes / physiology
Substances
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Antibodies, Monoclonal, Murine-Derived
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ICOSLG protein, human
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Immunoglobulin G
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Inducible T-Cell Co-Stimulator Ligand
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Pokeweed Mitogens