A novel anti-human ICOSL monoclonal antibody that enhances IgG production of B cells

Monoclon Antib Immunodiagn Immunother. 2013 Apr;32(2):125-31. doi: 10.1089/mab.2012.0121.

Abstract

ICOSL, a newly identified member of the B7 superfamily, plays a major role in immune responses. In this study, a functional anti-human ICOSL monoclonal antibody (MAb) 3B3 was obtained and characterized by means of flow cytometry, Western blot, and competition assay. This MAb could specifically recognize a distinct epitope of the ICOSL molecule. As a functional antibody, MAb 3B3 could inhibit the proliferation of T lymphocytes stimulated by ICOSL-L929 transfectants. Furthermore, it could enhance IgG production of PWM-driven B cells. The results indicate that the ICOS-ICOSL signal is critically involved in specific humoral immunity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal, Murine-Derived / pharmacology*
  • B-Lymphocytes / immunology*
  • B-Lymphocytes / metabolism
  • Binding, Competitive
  • Blotting, Western
  • Cell Line, Tumor
  • Cell Proliferation
  • Epitope Mapping
  • Humans
  • Hybridomas
  • Immunity, Humoral
  • Immunoglobulin G / biosynthesis*
  • Immunoglobulin G / immunology
  • Inducible T-Cell Co-Stimulator Ligand / antagonists & inhibitors
  • Inducible T-Cell Co-Stimulator Ligand / immunology*
  • Inducible T-Cell Co-Stimulator Ligand / metabolism
  • Mice
  • Mice, Inbred BALB C
  • Pokeweed Mitogens / immunology
  • Pokeweed Mitogens / pharmacology
  • Protein Binding
  • T-Lymphocytes / drug effects
  • T-Lymphocytes / physiology

Substances

  • Antibodies, Monoclonal, Murine-Derived
  • ICOSLG protein, human
  • Immunoglobulin G
  • Inducible T-Cell Co-Stimulator Ligand
  • Pokeweed Mitogens