Proteomic analysis of interstitial aortic valve cells acquiring a pro-calcific profile

Methods Mol Biol. 2013:1005:95-107. doi: 10.1007/978-1-62703-386-2_8.

Abstract

Cell-driven processes are now considered of relevance for the pathogenesis of aortic stenosis. In particular, during calcific valve degeneration, interstitial valve cells (VIC) resident in the leaflet can acquire an osteogenic/pro-calcific profile and actively contribute to matrix mineralization. The proteomic study described in this chapter is undertaken to investigate modifications in the proteome of bovine aortic VIC acquiring a calcifying phenotype. This approach can be useful to clarify cellular pathways involved in VIC pro-calcific differentiation and identify innovative therapeutic targets.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aortic Valve / metabolism*
  • Aortic Valve / pathology
  • Aortic Valve Stenosis / genetics
  • Aortic Valve Stenosis / metabolism*
  • Aortic Valve Stenosis / pathology
  • Calcinosis / genetics
  • Calcinosis / metabolism*
  • Calcinosis / pathology
  • Cattle
  • Cell Differentiation / genetics
  • Cells, Cultured
  • Electrophoresis, Gel, Two-Dimensional
  • Gene Expression
  • Muscle Cells / metabolism*
  • Muscle Cells / pathology
  • Peptide Mapping
  • Phenotype
  • Proteomics*
  • Signal Transduction
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Supplementary concepts

  • Aortic Valve, Calcification of