In the present study the influence of different conventional and reduced tillage systems onFusarium spp. and the production of the mycotoxin deoxynivalenol in winter wheat and maize in different crop rotation systems was investigated. Field trials were conducted in semihumid areas in Lower and Upper Austria and in the pannonian area in Eastern Austria. Generally, extremely dry and hot climatic conditions in all trial years resulted in low infestation levels. As a consequence, no significant effect of tillage system on the infestation withFusarium spp. in winter wheat respectively maize and the contamination with deoxynivalenol was found.