Production of zearalenone-4-glucoside, a-zearalenol-4-glucoside and ß-zearalenol-4-glucoside

P Krenn; F Berthiller; W Schweiger; C Hametner; R Ludwig; G Adam; R Krska; R Schuhmacher

doi:10.1007/BF02946045

Production of zearalenone-4-glucoside, a-zearalenol-4-glucoside and ß-zearalenol-4-glucoside

Mycotoxin Res. 2007 Dec;23(4):180-4. doi: 10.1007/BF02946045.

Authors

P Krenn¹, F Berthiller, W Schweiger, C Hametner, R Ludwig, G Adam, R Krska, R Schuhmacher

Affiliation

¹ Christian Doppler Laboratory for Mycotoxin Research, Dept. for Agrobiotechnology (IFA-Tulln), University of Natural Resources and Applied Life Sciences Vienna, Konrad Lorenz Str. 20, 3430, Tulln, Austria.

PMID: 23606022
DOI: 10.1007/BF02946045

Abstract

The work at hand describes the production of the zearalenone (ZON) metabolites zearalenone-4-glucoside (ZON-4G), a-zearalenol-4-glucoside (oc-ZOL-4G) and ß-zearalenol-4-glucoside (ß-ZOL-4G). In a first step a genetically modified yeast strain, expressing theArabidopsis thaliana UDP-glu-cosyltransferase UGT73C6, was treated with ZON to produce ZON-4G. The substance was purified by solid phase extraction and subsequent reversed phase preparative HPLC prior to the reduction with sodium borohydride to yield 0C-ZOL-4G and ß-ZOL-4G. The identity and purity of the substances were confirmed by(13)C-and(1)H-NMR as well as by HPLC-UV. In total, 50 mg of ZON were used to produce 5 mg of a-ZOL-4G with a purity of 98%, 6 mg of ß-ZOL-4G with a purity of 99% and 5 mg of ZON-4G with a purity of 99%.