Abstract
We investigated the effect of macrophage differentiation on the chemotactic activity to invade local damaged myoblasts using in vitro models of muscle injury. We found that: 1) the chemotactic activity of macrophages toward areas of damaged myoblasts might be induced more by live myoblasts than dead ones, 2) the chemotactic activity of macrophages is not due to velocity, but depends on the directionality toward damaged myoblast cells, and 3) macrophage differentiation strongly influence the chemotactic activity toward damaged myoblast cells through the expression of CCR2 and/or F-actin.
Copyright © 2013 Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Actins / immunology
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Actins / metabolism
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Animals
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Cell Differentiation / immunology
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Cell Line
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Chemokine CCL2 / immunology
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Chemokine CCL2 / metabolism
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Chemotaxis / immunology*
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Macrophages / immunology*
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Macrophages / metabolism
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Macrophages / pathology*
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Mice
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Muscle, Skeletal / immunology
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Muscle, Skeletal / metabolism
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Muscle, Skeletal / pathology
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Muscular Diseases / immunology
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Muscular Diseases / metabolism
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Muscular Diseases / pathology
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Myoblasts / immunology*
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Myoblasts / metabolism
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Myoblasts / pathology*
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Receptors, CCR2 / immunology
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Receptors, CCR2 / metabolism
Substances
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Actins
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Ccl2 protein, mouse
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Ccr2 protein, mouse
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Chemokine CCL2
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Receptors, CCR2