Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles

M Algarra; K Radotić; A Kalauzi; B Alonso; C M Casado; J C G Esteves da Silva

doi:10.1016/j.talanta.2012.09.008

Component analysis of fluorescence spectra of thiol DAB dendrimer/ZnSe-PEA nanoparticles

Talanta. 2013 Feb 15:105:267-71. doi: 10.1016/j.talanta.2012.09.008. Epub 2012 Sep 15.

Authors

M Algarra¹, K Radotić, A Kalauzi, B Alonso, C M Casado, J C G Esteves da Silva

Affiliation

¹ Centro de Geologia, Departamento de Geociências, Ambiente e Ordenamento do Território do Porto, Faculdade de Ciências da Universidade do Porto, Porto, Portugal. magonzal@fc.up.pt

PMID: 23598018
DOI: 10.1016/j.talanta.2012.09.008

Abstract

The fluorescence spectroscopy technique is an accurate method and has great utility in the interpretation of complex systems based on several emission bands. An interpretation of the system requires determination of the number, positions and intensities of the spectral components. In this work, the emission spectra of the synthesized ZnSe complex coated with O-phosphorylethanolamine (ZnSe-PEA), both with and without thiol DAB dendrimer generation 5 (S-DAB G5), were analyzed using a combination of asymmetric (log-normal) and symmetric (Gaussian) models. The method applied for the deconvolution of fluorescence spectra has proven to be very sensitive for observing the stability of the ZnSe-PEA complex after binding with S-DAB. The ZnSe-PEA emission spectrum contains two components. The positions of the emission maxima of these two components are not significantly affected by the presence of S-DAB G5 in the complex, which revealed the presence of a stable complex at a pH of 7. By applying the spectral deconvolution method, strong evidence was obtained that suggested that the ZnSe-PEA complex is stable after complexation with S-DAB G5.