Comparative gene expression analysis of the human periodontal ligament in deciduous and permanent teeth

Je Seon Song; Dong Hwan Hwang; Seong-Oh Kim; Mijeong Jeon; Byung-Jai Choi; Han-Sung Jung; Seok Jun Moon; Wonse Park; Hyung-Jun Choi

doi:10.1371/journal.pone.0061231

Comparative gene expression analysis of the human periodontal ligament in deciduous and permanent teeth

PLoS One. 2013 Apr 8;8(4):e61231. doi: 10.1371/journal.pone.0061231. Print 2013.

Authors

Je Seon Song¹, Dong Hwan Hwang, Seong-Oh Kim, Mijeong Jeon, Byung-Jai Choi, Han-Sung Jung, Seok Jun Moon, Wonse Park, Hyung-Jun Choi

Affiliation

¹ Department of Pediatric Dentistry, College of Dentistry, Yonsei University, Seoul, Korea.

Abstract

There are histological and functional differences between human deciduous and permanent periodontal ligament (PDL) tissues. The aim of this study was to determine the differences between these two types of tissue at the molecular level by comparing their gene expression patterns. PDL samples were obtained from permanent premolars (n = 38) and anterior deciduous teeth (n = 31) extracted from 40 healthy persons. Comparative cDNA microarray analysis revealed several differences in gene expression between the deciduous and permanent PDL tissues. These findings were verified by qRT-PCR (quantitative reverse-transcription-polymerase chain reaction) analysis, and the areas where genes are expressed were revealed by immunohistochemical staining. The expressions of 21 genes were up-regulated in deciduous relative to PDL tissues, and those of 30 genes were up-regulated in permanent relative to deciduous PDL tissues. The genes that were up-regulated in deciduous PDL tissues were those involved in the formation of the extracellular matrix (LAMC2, LAMB3, and COMP), tissue development (IGF2BP, MAB21L2, and PAX3), and inflammatory or immune reactions leading to tissue degradation (IL1A, CCL21, and CCL18). The up-regulated genes in permanent PDL tissues were related to tissue degradation (IL6 and ADAMTS18), myocontraction (PDE3B, CASQ2, and MYH10), and neurological responses (FOS, NCAM2, SYT1, SLC22A3, DOCK3, LRRTM1, LRRTM3, PRSS12, and ARPP21). The analysis of differential gene expressions between deciduous and permanent PDL tissues aids our understanding of histological and functional differences between them at the molecular level.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adolescent
Child
Child, Preschool
Female
Gene Expression Profiling*
Humans
Male
Periodontal Ligament / cytology
Periodontal Ligament / metabolism*
Tooth, Deciduous / cytology
Tooth, Deciduous / metabolism*
Young Adult

Grants and funding

This research was supported by Basic Science Research Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2011-0022160 and 2012R1A1A2041910). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.