Abstract
Don't interrupt! Protein serotonylation has been implicated in living cells, yet its role remains poorly defined because of the lack of characterization tools. We synthesized a serotonin derivative to enable selective tagging of serotonylation and to investigate its effect on Ras; the latter displayed undisrupted interaction with Raf-1 at the Ras binding domain.
Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Cell Line, Tumor
-
Enzyme Activation
-
Humans
-
Protein Processing, Post-Translational*
-
Serotonin / analogs & derivatives
-
Serotonin / metabolism*
-
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
-
Tandem Mass Spectrometry
-
Transglutaminases / metabolism*
-
ras Proteins / chemistry
-
ras Proteins / metabolism*
Substances
-
Serotonin
-
Transglutaminases
-
ras Proteins