Comparison of influenza virus replication fidelity in vitro using selection pressure with monoclonal antibodies

J Med Virol. 2013 Jun;85(6):1090-4. doi: 10.1002/jmv.23532.

Abstract

The replication fidelity of reassortant A/Fujian/411/02(H3N2)-like influenza viruses was assessed by in vitro detection of escape mutants developing under selective pressure from monoclonal antibodies. The results showed A/Wyoming/3/03(H3N2) possessed lower fidelity relative to the A/California/7/04(H3N2) and A/Wisconsin/57/05(H3N2) viruses through the emergence of mutant viruses carrying H156Q hemagglutinin mutation which allows antibody escape. Using the neutralization assay to compare the fidelity of reassortant pandemic A/California/7/09(H1N1) viruses, the pandemic virus was shown to possess relative higher fidelity compared to A/Wyoming/3/03. This higher fidelity may contribute to the lack of major antigenic changes in the pandemic virus since the emergence in 2009.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / genetics*
  • Antibodies, Viral / genetics*
  • Dogs
  • Genetic Engineering
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics
  • Hemagglutinin Glycoproteins, Influenza Virus / immunology*
  • Humans
  • Immune Evasion
  • Influenza A Virus, H3N2 Subtype / genetics
  • Influenza A Virus, H3N2 Subtype / immunology*
  • Madin Darby Canine Kidney Cells
  • Models, Molecular
  • Mutation
  • Neuraminidase / genetics
  • Neuraminidase / immunology
  • Neutralization Tests
  • Reassortant Viruses / genetics
  • Reassortant Viruses / immunology*
  • Virus Replication*

Substances

  • Antibodies, Monoclonal
  • Antibodies, Viral
  • Hemagglutinin Glycoproteins, Influenza Virus
  • hemagglutinin, human influenza A virus
  • Neuraminidase