Extremely selective "turn-on" fluorescence detection of hypochlorite confirmed by proof-of-principle neurological studies via esterase action in living cells

Analyst. 2013 May 21;138(10):2829-32. doi: 10.1039/c3an00297g.

Abstract

Highly specific and sensitive fluorescence detection of hypochlorite in nonbiotic pure water (rapid "turn-on", ~400 fold, λ(em) ~ 560 nm) as well as in living neuronal cell cultures (neutral pH) involves oxidation of a 2-sulfide-2-benzoic acid pendent group in a new meso-thienyl-BODIPY donor-acceptor probe.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzenesulfonates / chemistry
  • Benzoates / chemistry
  • Boron Compounds / chemistry*
  • Cell Survival
  • Esterases / metabolism*
  • Fluorescence*
  • Fluorescent Dyes / chemistry*
  • Humans
  • Hypochlorous Acid / analysis*
  • Microscopy, Fluorescence
  • Molecular Structure
  • Neuroblastoma / enzymology*
  • Neuroblastoma / metabolism
  • Neuroblastoma / pathology
  • Oxidation-Reduction
  • Reactive Oxygen Species / metabolism
  • Tumor Cells, Cultured

Substances

  • 4,4-difluoro-4-bora-3a,4a-diaza-s-indacene
  • Benzenesulfonates
  • Benzoates
  • Boron Compounds
  • Fluorescent Dyes
  • Reactive Oxygen Species
  • Hypochlorous Acid
  • Esterases
  • 2-sulfobenzoic acid