Pyrrolizidine alkaloids from Liparis nervosa with inhibitory activities against LPS-induced NO production in RAW264.7 macrophages

Phytochemistry. 2013 Sep:93:154-61. doi: 10.1016/j.phytochem.2013.03.009. Epub 2013 Apr 6.

Abstract

Six pyrrolizidine alkaloids were isolated from the whole herb of Liparis nervosa together with two previously known ones. Their structures were elucidated by extensive spectroscopic analyses and chemical reactions. The cytotoxicity of the isolates was evaluated against A549, HepG2, and MCF-7 human cancer cell lines; however, no significant growth inhibition was observed. All compounds were evaluated for the inhibition of LPS-induced nitric oxide (NO) production in RAW264.7 macrophages, and most significantly inhibited NO production with IC50 values in the range of 2.16-38.25 μM.

Keywords: Liparis nervosa; NO production inhibitor; Nervogenic acid derivatives; Nervosine; Orchidaceae; Pyrrolizidine alkaloids.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Survival / drug effects
  • Dose-Response Relationship, Drug
  • Hep G2 Cells
  • Humans
  • Lipopolysaccharides / antagonists & inhibitors*
  • Lipopolysaccharides / pharmacology
  • MCF-7 Cells
  • Macrophages / drug effects*
  • Macrophages / metabolism
  • Mice
  • Molecular Conformation
  • Nitric Oxide / antagonists & inhibitors*
  • Nitric Oxide / biosynthesis
  • Orchidaceae / chemistry*
  • Pyrrolizidine Alkaloids / chemistry
  • Pyrrolizidine Alkaloids / isolation & purification
  • Pyrrolizidine Alkaloids / pharmacology*
  • Structure-Activity Relationship
  • Tumor Cells, Cultured

Substances

  • Lipopolysaccharides
  • Pyrrolizidine Alkaloids
  • Nitric Oxide