Aptamer-conjugated magnetic nanoparticles enable efficient targeted detection of integrin αvβ3 via magnetic resonance imaging

J Biomed Mater Res A. 2014 Jan;102(1):49-59. doi: 10.1002/jbm.a.34678. Epub 2013 Apr 9.

Abstract

An understanding of neovascularization and/or angiogenesis in cancer is acutely required for effective cancer therapy due to concerns about tumor growth and metastasis. In particular, integrin αvβ3 is closely associated with cell migration and invasion during angiogenesis. Hence, we developed aptamer(αvβ3)-conjugated magnetic nanoparticles (Apt(αvβ3)-MNPs) to enable precise detection of integrin-expressing cancer cells using magnetic resonance imaging. Apt(αvβ3)-MNPs exhibited not only cytocompatibility, but also an efficient targeting ability with high magnetic sensitivity through in vitro/in vivo studies. The results of this study demonstrate that Apt(αvβ3)-MNPs have the potential to be used for accurate tumor diagnosis and therapy.

Keywords: angiogenesis; aptamer; integrin avb3; magnetic nanoparticle; magnetic resonance imaging.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aptamers, Peptide* / chemistry
  • Aptamers, Peptide* / pharmacology
  • Contrast Media* / chemistry
  • Contrast Media* / pharmacology
  • Drug Delivery Systems / methods*
  • Humans
  • Integrin alphaVbeta3 / chemistry*
  • Magnetic Fields*
  • Magnetic Resonance Angiography*
  • Male
  • Mice
  • Mice, Inbred BALB C
  • Mice, Nude
  • Nanoparticles / chemistry*
  • Neoplasms, Experimental* / blood supply
  • Neoplasms, Experimental* / diagnostic imaging
  • Neoplasms, Experimental* / metabolism
  • Neovascularization, Pathologic / diagnostic imaging*
  • Radiography

Substances

  • Aptamers, Peptide
  • Contrast Media
  • Integrin alphaVbeta3