Rapid purification of Soybean mosaic virus from small quantities of tissue by immunoprecipitation

Jang-Kyun Seo; Minji Kang; Mi Sa Vo Phan; Kook-Hyung Kim

doi:10.1016/j.jviromet.2013.03.026

Rapid purification of Soybean mosaic virus from small quantities of tissue by immunoprecipitation

J Virol Methods. 2013 Jul;191(1):31-2. doi: 10.1016/j.jviromet.2013.03.026. Epub 2013 Apr 6.

Authors

Jang-Kyun Seo¹, Minji Kang, Mi Sa Vo Phan, Kook-Hyung Kim

Affiliation

¹ Department of Agricultural Biotechnology and Plant Genomics and Breeding Institute, Seoul National University, Seoul 151-921, Republic of Korea.

PMID: 23567843
DOI: 10.1016/j.jviromet.2013.03.026

Abstract

A rapid, simple, and efficient method for purification of Soybean mosaic virus (SMV) was developed based on immunoprecipitation. Traditional centrifugation-based methods for purification of SMV and other potyviruses require long, complicated procedures and large quantities of infected tissue (100-500 g). The immunoprecipitation procedure described in this study allows the purification of intact SMV virion particles in 4h from 0.5 g of tissue. The reliability of this procedure was demonstrated by RT-PCR and transmission electron microscopy (TEM). This method will be useful for high-throughput examination of the physical and morphological properties of virus particles because intact virion preparations ready for TEM observation can be purified rapidly from very small tissue samples.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Glycine max / virology*
Immunoprecipitation / methods*
Microscopy, Electron, Transmission
Potyvirus / isolation & purification*
Reverse Transcriptase Polymerase Chain Reaction
Virology / methods*