Microbial production of 2,3 butanediol from seaweed hydrolysate using metabolically engineered Escherichia coli

Abstract

A variety of biofuel and biorefinery products have been produced from engineered Escherichia coli till date. Most of these products had been derived from simple sugars in its pure form, rather than deriving it from alternative, renewable and carbon neutral sources, such as marine alga biomass. Engineering E. coli to use algal hydrolysate can make these an attractive carbon source for the industrial production of value added fuels and chemicals. This work reports the engineering of E. coli by a combination of gene deletion and synthetic pathway incorporation, for the efficient utilization of algal hydrolysate to produce BA (2,3 butanediol+acetoin) under microaerobic condition. Engineered strain produced ~19 g/L of total BA from algal hydrolysate in defined M9 salt media at a yield of 0.43 g/g.