The immunotoxicity of graphene oxides and the effect of PVP-coating

Xiao Zhi; Hongliang Fang; Chenchen Bao; Guangxia Shen; Jiali Zhang; Kan Wang; Shouwu Guo; Tao Wan; Daxiang Cui

doi:10.1016/j.biomaterials.2013.03.024

The immunotoxicity of graphene oxides and the effect of PVP-coating

Biomaterials. 2013 Jul;34(21):5254-61. doi: 10.1016/j.biomaterials.2013.03.024. Epub 2013 Apr 6.

Authors

Xiao Zhi¹, Hongliang Fang, Chenchen Bao, Guangxia Shen, Jiali Zhang, Kan Wang, Shouwu Guo, Tao Wan, Daxiang Cui

Affiliation

¹ Department of Bio-Nano Science and Engineering, Key Laboratory for Thin Film and Microfabrication of Ministry of Education, Institute of Micro/Nano Science and Technology, Shanghai Jiao Tong University, Shanghai 200240, People's Republic China.

PMID: 23566800
DOI: 10.1016/j.biomaterials.2013.03.024

Abstract

Graphene oxide (GO) immunotoxicity is not clarified well up to date. Herein we reported the effects of GOs with and without polyvinylpyrrolidone (PVP) coating on human immune cells such as dendritic cells (DCs), T lymphocytes and macrophages. Human immune cells such as dendritic cells (DCs), T lymphocytes and macrophages were isolated from health donated bloods, PVP-coating GO (PVP-GO) exhibited lower immunogenicity compared with pure GO on the aspect of inducing differentiation and maturation of dendritic cells (DCs), the levels of secreted TNF-α and IL-1β had no obvious difference between two groups, yet the secretion of IL-6 remained in PVP-coating GO group. In addition, PVP-coating GO delayed significantly the apoptotic process of T lymphocytes, at the same time, and exhibited anti-phagocytosis ability against macrophages and markedly enhanced the physiological activity of macrophages. In conclusion, PVP-coating GO possesses good immunological biocompatibility and immunoenhancement effects in vitro, and is likely to be an available candidate of immunoadjuvant in the future.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Apoptosis / drug effects
Coated Materials, Biocompatible / pharmacology*
Cytokines / metabolism
Dendritic Cells / cytology
Dendritic Cells / drug effects
Dendritic Cells / metabolism
Graphite / chemistry*
Graphite / toxicity*
Humans
Immunotoxins / toxicity*
Macrophages / cytology
Macrophages / drug effects
Macrophages / metabolism
Microscopy, Atomic Force
Mitochondria / drug effects
Mitochondria / metabolism
Models, Biological
Oxides / chemistry*
Phenotype
Povidone / pharmacology*
T-Lymphocytes / cytology
T-Lymphocytes / drug effects
T-Lymphocytes / metabolism

Substances

Coated Materials, Biocompatible
Cytokines
Immunotoxins
Oxides
Graphite
Povidone