Abstract
This paper describes the development of nucleic acid detection in paper using a combination of commercial fluorescent probes and DNA ladders, and provides us with a better understanding of the interactions between double-stranded DNA (the amplified products in this study), fiber structures in paper, and fluorescent probes. The amplified products (the reverse-transcription and amplification of dengue virus serotype-2 RNA via RT-LAMP) in this study were subsequently fluorescently labeled in paper-based test zones (on our paper-based diagnostic device), thus fluorescent probes were used to perform the diagnosis of dengue fever, specific to serotype-2.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA, Viral / genetics
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DNA, Viral / isolation & purification
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Dengue / virology*
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Dengue Virus / classification
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Dengue Virus / genetics
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Dengue Virus / isolation & purification*
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Equipment Design
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Fluorescent Dyes / chemistry
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Humans
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Image Processing, Computer-Assisted
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Molecular Diagnostic Techniques / instrumentation
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Molecular Diagnostic Techniques / methods*
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Nucleic Acid Amplification Techniques / instrumentation
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Nucleic Acid Amplification Techniques / methods*
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Oligonucleotide Probes / genetics
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Paper*
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RNA, Viral / genetics
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RNA, Viral / isolation & purification
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Reverse Transcriptase Polymerase Chain Reaction / instrumentation
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Reverse Transcriptase Polymerase Chain Reaction / methods
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Sensitivity and Specificity
Substances
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DNA, Viral
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Fluorescent Dyes
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Oligonucleotide Probes
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RNA, Viral