Improvement of the lipophilic-oxygen radical absorbance capacity (L-ORAC) method and single-laboratory validation

Biosci Biotechnol Biochem. 2013;77(4):857-9. doi: 10.1271/bbb.120786. Epub 2013 Apr 7.

Abstract

We improved the procedure for lipophilic-oxygen radical absorbance capacity (L-ORAC) measurement for better repeatability and intermediate precision. A sealing film was placed on the assay plate, and glass vials and microdispensers equipped with glass capillaries were used. The antioxidant capacities of food extracts can be evaluated by this method with nearly the same precision as antioxidant solutions.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Free Radical Scavengers / chemistry*
  • Free Radicals / chemistry
  • Hydrophobic and Hydrophilic Interactions*
  • Laboratories*
  • Reproducibility of Results
  • Spectrometry, Fluorescence / methods*

Substances

  • Free Radical Scavengers
  • Free Radicals