Abstract
The bio-nanocapsules displaying about 240 molecules of immunoglobulin G Fc-binding Z domains (ZZ-BNCs) enhanced the signals of enzyme-linked immunosorbent assay by tethering the Fc regions of secondary antibodies (Abs), which were eliminated using high-molecular mass enzymes (e.g., alkaline phosphatase). By way of optimizing the distance between enzymes and Abs, ZZ-BNCs improved sensitivity independently of enzymes.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkaline Phosphatase / chemistry
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Alkaline Phosphatase / metabolism*
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Enzyme-Linked Immunosorbent Assay / methods*
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Horseradish Peroxidase / metabolism
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Immunoglobulin Fc Fragments / chemistry
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Immunoglobulin Fc Fragments / immunology
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Immunoglobulin G / chemistry
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Immunoglobulin G / immunology
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Liposomes / chemistry
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Molecular Weight
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Nanocapsules*
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Protein Structure, Tertiary
Substances
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Immunoglobulin Fc Fragments
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Immunoglobulin G
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Liposomes
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Nanocapsules
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Horseradish Peroxidase
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Alkaline Phosphatase