Surface plasmon resonance sensing of nucleic acids: a review

Anal Chim Acta. 2013 Apr 22:773:9-23. doi: 10.1016/j.aca.2012.12.040. Epub 2013 Jan 2.

Abstract

Biosensors based on surface plasmon resonance (SPR) have become a central tool for the investigation and quantification of biomolecules and their interactions. Nucleic acids (NAs) play a vital role in numerous biological processes and therefore have been one of the major groups of biomolecules targeted by the SPR biosensors. This paper discusses the advances of NA SPR biosensor technology and reviews its applications both in the research of molecular interactions involving NAs (NA-NA, NA-protein, NA-small molecule), as well as for the field of bioanalytics in the areas of food safety, medical diagnosis and environmental monitoring.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Biosensing Techniques
  • Environmental Monitoring
  • Food Analysis
  • HIV / genetics
  • Humans
  • Immobilized Nucleic Acids / chemistry
  • Immobilized Nucleic Acids / metabolism
  • Nucleic Acids / analysis*
  • Oligonucleotide Array Sequence Analysis
  • Oligonucleotide Probes / chemistry
  • Oligonucleotide Probes / metabolism
  • Poly(ADP-ribose) Polymerases / metabolism
  • Surface Plasmon Resonance*

Substances

  • Immobilized Nucleic Acids
  • Nucleic Acids
  • Oligonucleotide Probes
  • Poly(ADP-ribose) Polymerases