Abstract
We describe an innovation in the immobilization, culture, and imaging of cells in calcium alginate within microfluidic devices. This technique allows unprecedented optical access to the entirety of the calcium alginate hydrogel, enabling observation of growth and behavior in a chemical and mechanical environment favored by many kinds of cells.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Alginates / chemistry*
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Calcium / chemistry
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Electrodes
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Epithelial Cells / cytology
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Epithelial Cells / metabolism
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Escherichia coli / cytology
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Escherichia coli / metabolism
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Glucuronic Acid / chemistry
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Hexuronic Acids / chemistry
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Humans
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Hydrogels / chemistry*
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Microfluidic Analytical Techniques / instrumentation
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Microfluidic Analytical Techniques / methods*
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Microscopy, Fluorescence
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Surface Properties
Substances
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Alginates
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Hexuronic Acids
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Hydrogels
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Glucuronic Acid
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Calcium