Abstract
We report herein a simple and convenient luminescent assay for detection of base excision repair enzyme activity using an Ir(III) complex as a G-quadruplex selective probe. Using uracil-DNA glycosylase (UDG) as a model enzyme, the assay achieved high sensitivity and selectivity for UDG over other tested enzymes. The utility of the assay for screening potential UDG inhibitors was also demonstrated.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Biological Assay
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Biosensing Techniques
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Coordination Complexes / chemistry
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DNA Repair
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G-Quadruplexes*
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Iridium / chemistry
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Luminescence
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Luminescent Measurements
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Molecular Probe Techniques
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Uracil-DNA Glycosidase / antagonists & inhibitors
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Uracil-DNA Glycosidase / chemistry
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Uracil-DNA Glycosidase / metabolism*
Substances
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Coordination Complexes
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Iridium
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Uracil-DNA Glycosidase